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Genetic Engineering

Code:

BIOT158

Sigla:

EG

Áreas Científicas
Classificação	Área Científica
OFICIAL	Biotecnologia

Ocorrência: 2019/2020 - 1S

Ativa?	Yes
Unidade Responsável:	Biotecnologia
Curso/CE Responsável:	Undergraduate in Biotechnology

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
BIOT	47	Study Plan	3	-	4,5	52,5	121,5

Língua de trabalho

Portuguese

Objetivos

1.Understand the fundamentals of genetic engineering
2.Understanding the potential of biotechnology of plants, animals and microorganisms in genetic engineering

Resultados de aprendizagem e competências

3.Knowledge about common methodologies in genetic engineering
4.Apply tools of recombinant DNA and cloning in bacteria

Modo de trabalho

Presencial

Programa

1.Biotechnology and Genetic Engineering
2.Recombinant DNA technology
2.1.Enzymes
2.2.Recombinant DNA molecules
2.3.PCR technique
2.4.Methods for automatic sequencing
2.5.DNA libraries
2.6.Applications
3.Gene expression systems
3.1.Host models
3.2.Vectors
3.3.Gene clonning
3.4.Applications
4.Transgenic models
4.1.Animals and plants
4.2.Knocking out gene function
4.3.Applications
5.Gene and protein technology
5.1.Vectors
5.2.Sites for gene therapy
6.Reproductive technologies
6.1.Fertilization and cryopreservation techniques
6.2.Gamete transfer

Bibliografia Obrigatória

T. A. Brown; Gene cloning and DNA analysis : an introduction, Wiley Blackwell, , 2016. ISBN: 978-1-119-07256-0
Desmond S. T. Nicholl; An introduction to genetic engineering, Cambridge University Press, 2008. ISBN: 978-0-521-61521-1
A. J. Nair; Introduction to biotechnology and genetic engineering, Jones & Bartlett Learning, 2008. ISBN: 978-0-7637-7375-5
Keith Wilson, John Walker; Principles and techniques of biochemistry and molecular biology, Cambridge University Press, 2010. ISBN: 978-0-521-73167-6
Michael R. Green, Joseph sambrook; Molecular cloning : a laboratory manual, CSH Press, 2012. ISBN: 978-193611342-2
Benjamin Lewin; Genes, John Wiley & Sons

Métodos de ensino e atividades de aprendizagem

The theoretical component will be presented in expository mode using Powerpoint slides. In theoretical-practical classes, more practical aspects of the syllabus will be worked through by solving exercises. The integration of concepts will also be carried out through the elaboration of a genetic engineering project (designing a cloning strategy with a specific objective) that is implemented in the accompannying curricular unit Laboratory VB.

Software

ensembl.org e ncbi.nlm.nih.gov (bases de dados online)
Benchling
Lasergene

Palavras Chave

Natural sciences > Biological sciences > Biology > Genetics
Natural sciences > Biological sciences > Biological engineering > Genetic engineering

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	60,00
Trabalho escrito	40,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Elaboração de projeto	15,00
Estudo autónomo	30,00
Frequência das aulas	45,00
Trabalho de investigação	15,00
Trabalho escrito	16,50
Total:	121,50

Obtenção de frequência

Regarding the attendance regime, access to the continuous assessment regime is denied to the student who presents a value greater than 40% of unexcused absences from all classes of the semester. It is the RUC that decides, on a case-by-case basis, whether to accept the justification for the absence.

Fórmula de cálculo da classificação final

Continuous assessment:
FC = 75 % (average of 2 tests) + 25% (project)

Exam(s):
FC = 100 % Exam OR 75% Exam + 25% (project) – the best of the 2 options.

ot having a minimum grade alone, the average of the 2 continuous assessment tests is 9.5. The minimum grade in the exam is also 9.5 val.

Provas e trabalhos especiais

The work is mandatory exclusively for access to continuous assessment. It consists of the development of a genetic engineering project, with the elaboration of the laboratory protocol that the students will develop, in a PBL conjugated with the UC of Laboratórios VB, and in the oral and poster presentation of the final results.

The cloning project will be partially elaborated in class, as a way of contextualizing the theoretical concepts and their application to solve a given problem. The evolution of the project will be monitored throughout the semester, establishing intermediate “deadlines” to complete specific stages. The scheduling of the steps to be developed in the project assumes that the protocol is completed in time for the laboratory class of its implementation, with the delivery of the final poster scheduled for the end of the academic period, as it combines the results obtained in the UC of Laboratórios VB.

The evaluation is made by the commitment and autonomy of the project and protocol development (25%), delivery and quality of the protocol (25%), presentation and analysis of the results obtained in the laboratory execution, in poster format (25%) and oral presentation of the poster/discussion (25%)

Avaliação especial (TE, DA, ...)

There is no provision for any special assessment regime, not included in those already provided for in the Regulation.