Autenticação

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Cell and Tissue Technology

Code:

MEBQB10

Sigla:

TCT

Áreas Científicas
Classificação	Área Científica
OFICIAL	Biotecnologia

Ocorrência: 2019/2020 - 2S

Ativa?	Yes
Unidade Responsável:	Biotecnologia
Curso/CE Responsável:	Master in Biological and Chemical Engineering

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
MEBQ	18	Study Plan	1	-	5	52,5	135

Língua de trabalho

Portuguese

Objetivos

Provide students with an integrated education of cell and tissue technology, based on fundamental concepts of Cellular and Molecular Biology, Genetics, Immunology and bio-process, with applications in human and veterinary medicine.

Resultados de aprendizagem e competências

Not Applicable

Modo de trabalho

À distância

Programa

1. Introduction to Animal and Tissue technology: history; differences between animal cells and microorganisms; examples of animal cells applications in commercial production


2. Basics concepts on Animal Cells: typical structure of animal cells; type of cellular cultures; cellular growth and propagation; cryopreservation; storage; quality control and safety; principal cell lines used in the industry; insect cells


3. Cloning and heterologous expression in animal cells: genetic flow of information and molecular cloning; elements that are needed for the genetic expression in eukaryotic cells; heterologous expression systems in animal cells; viral DNA vectors; viral RNA vectors; baculovirus; plasmidic vectors; cells lines in biotechnological processes; expression in animal cells; DNA transfection in animal cells; selection markers


4. Cell Metabolism and its control: energy sources; metabolic bioproducts; factors affecting the cellular metabolism


5. Post-translational modification of recombinant proteins


6. Mechanisms of cells proliferation and death


7. Culture media for animal cells


8. Bioreactors for animal cells: bioreactors project and operation; cellular kinetics; modelling; monitorization and control of cell cultures


9. Animal cells separation and purification methods


10. Cells therapy and stem cells


11. Gene therapy: case study – adenoviral vectors production (for vaccination and gene therapy)


12. Tissue Microfabrication.

Bibliografia Obrigatória

Leda R. Castilho, ângela Maria Moraes, Elisabeth F.P. Augusto and Michael Butler; Animal Cell Technology: From Biopharmaceuticals to Gene Therapy
R. Ian Freshney; Culture of Animal Cells
Clemens van Blitterswijk, Peter Thomsen, Anders Lindahl, Jeffrey Hubbell, David Williams, Ranieri Cancedda, Joost de Bruijn, Jerôme Sohier; Tissue Engineering
Palsson, B.Ø., Bhatia, S.N., ; Tissue Engineering
C. van Blitterswijk; Tissue Engineering
Doyle, A., Griffiths, J.B.; Cell and Tissue Culture
Lanza, R., Langer, R. e Vacanti, J.; Principles of Tissue Engineering
Koller, M., Palsson, B. e Masters, J.; Human Cell Culture, Vol. IV, Primary Hematopoietic Cells
Marshak, D., Gardner, R., Gottlieb, D.; Stem Cell Biology
Atala, A., Lanza, R., Thomson, J., Nerem, R.; Principles of Regenerative Medicine

Métodos de ensino e atividades de aprendizagem

For the Academic year 2019-2020, the classes were performed remotely

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Apresentação/discussão de um trabalho científico	100,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Frequência das aulas	52,50
Estudo autónomo	82,50
Total:	135,00

Obtenção de frequência

Not Aplicable

Fórmula de cálculo da classificação final

Continuous evaluation:

NF= 50% Work 1+ 50% Work 2


Exam (all phases):

NF=100% Exam