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Biochemistry and Biochemical Analysis

Code:

TLQB015

Sigla:

BAB

Áreas Científicas
Classificação	Área Científica
CNAEF	Biology and Biochemistry

Ocorrência: 2020/2021 - 1S

Ativa?	Yes
Unidade Responsável:	Biotecnologia
Curso/CE Responsável:	Professional Higher Technical Course in Chemical and Biological Laboratory Technologies

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
TLQB	34	Study Plan	2	-	7,5	75	202,5

Língua de trabalho

Portuguese
Obs.: Português suitable for english speaken students

Objetivos

At the end of the curricular unit (CU), the student is expected to have theoretical knowledge about: - the structure and organization of different cell constituents in different types of cells; - the function and structure of the most important biomolecules, namely nucleic acids (RNA, DNA), carbohydrates, lipids and proteins; - have notions of enzymatic kinetics and enzymatic assays.

They must also have theoretical knowledge and know how to apply in a laboratory context: - preparation of buffer solutions typical of biochemical assays, - biomolecule purification techniques, namely, chromatographic and ultrafiltration techniques, - biomolecules assay techniques (glycosides, proteins, nucleic acids ), mastering colorimetric methods and spectrophotometric analysis, - analytical techniques for protein separation (acrylamide gel electrophoresis), - analysis and assay methods based on direct and indirect enzymatic assays.

Resultados de aprendizagem e competências

Not applicable

Modo de trabalho

Presencial

Programa

Theoretical Contents:

1. Biodiversity of living organisms; different types of cells and their characteristics


2. Function and structure of biomolecules: proteins, nucleic acids (DNA and RNA), carbohydrates, lipids.


3. Protein Synthesis (transcription and translation)


4. Enzyme kinetics (specific enzymatic activity, Michaelis Menten kinetics, enzymatic assays)

Laboratory Application Contents

1. Spectrophotometric methods: determination of nucleic acids or proteins by UV absorption and by quantitative colorimetric methods.


2. Preparative methods of separating biomolecules:

• Dialysis; centrifugation, ultracentrifugation and gradient centrifugation.


• Filtration and ultrafiltration techniques and chromatographic methods: Hydrophobic, ion exchange, affinity and molecular exclusion interactions. Application in the isolation and purification of proteins and nucleic acids.


• Electrophoretic methods. Separation of proteins on an SDS-PAGE gel and nucleic acids on an agarose gel.

Bibliografia Obrigatória

D. L. Nelson; M. M. Cox ; Lehninger´s Principles of Biochemistry, W. H. Freeman and Company, 2012. ISBN: 9780716743392

Bibliografia Complementar

J. M. Berg; J. L. Tymoczko; L. Stryer ; Biochemistry, Stryer , W. H. Freeman and Company, 2006. ISBN: 9780716767664
D. Voet ; Biochemistry , Voet , Wiley, John Wiley & Sons, 2010. ISBN: 978-0470570951
A. Quintas ; Bioquímica : Organização Molecular da Vida , Lidel, 2008. ISBN: 978972757431

Métodos de ensino e atividades de aprendizagem

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	50,00
Trabalho laboratorial	50,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	127,50
Frequência das aulas	37,50
Trabalho laboratorial	37,50
Total:	202,50

Obtenção de frequência

Assessment in the theoretical component: three written tests (weight of 15% 1st test; 15% 2nd test and 20% for the 3rd test)

No minimum score is imposed for each test, separately, but its weighted average must be greater than 9.5.

Evaluation in the laboratory component:, carried out through:

- Classroom context and laboratory notebook 10%

- Assignment 1: Individual online quizzes and discussion 20%

- Assignment 2: Reports of practical laboratory assignments 20%

The weighted average of the three classifications has a minimum score of 9.5. No minimum grade is imposed for each assessment element

Fórmula de cálculo da classificação final

Theoretical component (TC) 

TC: 0.15* 1st test+0.15* 2nd test+0.20*3rd test

Practical Component (PC)

PC: 0.10 x classroom context +0.20 x Assignment 1 + 0.20 x Assignment 2

Final Grade= TC + PC

The non-approval of the practical component makes it impossible to approve the CU in the current year

Exam only for the theoretical component (TC)

100%*exam grade

Observações

The tests performed in the theoretical-practical component do not have a minimum grade, but their average must be equal to or greater than 9.5.

The non-validation of the laboratory component (final classification of 9.5) invalidates the performance of exams and approval in the UC


Laboratory classes were held in person. the theoretical-practical classes were held at a distance.