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Genetic Engineering

Code:

BIOT158

Sigla:

EG

Áreas Científicas
Classificação	Área Científica
OFICIAL	Biotecnologia

Ocorrência: 2022/2023 - 1S

Ativa?	Yes
Página Web:	https://moodle.ips.pt/2223/course/view.php?id=1736
Unidade Responsável:	Departamento de Engenharia Química e Biológica
Curso/CE Responsável:	Undergraduate in Biotechnology

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
BIOT	68	Study Plan	3	-	4,5	52,5	121,5

Língua de trabalho

Portuguese

Objetivos

1.Understand the fundamentals of genetic engineering
2.Understanding the potential of biotechnology of plants, animals and microorganisms in genetic engineering

Resultados de aprendizagem e competências

3.Knowledge about common methodologies in genetic engineering
4.Apply tools of recombinant DNA and cloning in bacteria

Modo de trabalho

Presencial

Programa

1.Biotechnology and Genetic Engineering
2.Recombinant DNA technology
2.1.Enzymes
2.2.Recombinant DNA molecules
2.3.PCR technique
2.4.Methods for automatic sequencing
2.5.DNA libraries
2.6.Applications
3.Gene expression systems
3.1.Host models
3.2.Vectors
3.3.Gene clonning
3.4.Applications
4.Transgenic models
4.1.Animals and plants
4.2.Knocking out gene function
4.3.Applications
5.Gene and protein technology
5.1.Vectors
5.2.Sites for gene therapy
6.Reproductive technologies
6.1.Fertilization and cryopreservation techniques
6.2.Gamete transfer

Bibliografia Obrigatória

T. A. Brown; Gene cloning and DNA analysis : an introduction, Wiley Blackwell, , 2016. ISBN: 978-1-119-07256-0
Desmond S. T. Nicholl; An introduction to genetic engineering, Cambridge University Press, 2008. ISBN: 978-0-521-61521-1
A. J. Nair; Introduction to biotechnology and genetic engineering, Jones & Bartlett Learning, 2008. ISBN: 978-0-7637-7375-5
Keith Wilson, John Walker; Principles and techniques of biochemistry and molecular biology, Cambridge University Press, 2010. ISBN: 978-0-521-73167-6
Michael R. Green, Joseph sambrook; Molecular cloning : a laboratory manual, CSH Press, 2012. ISBN: 978-193611342-2
Benjamin Lewin; Genes, John Wiley & Sons

Métodos de ensino e atividades de aprendizagem

The theoretical component will be presented in expository mode using Powerpoint slides. In theoretical-practical classes, more practical aspects of the syllabus will be worked through by solving exercises. The integration of concepts will also be carried out through the elaboration of a small genetic engineering project (designing a cloning strategy with a specific objective).

Software

Benchling
ensembl.org e ncbi.nlm.nih.gov (bases de dados online)
Lasergene

Palavras Chave

Natural sciences > Biological sciences > Biology > Genetics
Natural sciences > Biological sciences > Biology > Molecular biology
Natural sciences > Biological sciences > Biological engineering > Genetic engineering

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	90,00
Trabalho escrito	10,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Elaboração de projeto	10,00
Estudo autónomo	46,50
Frequência das aulas	45,00
Trabalho de investigação	10,00
Trabalho escrito	10,00
Total:	121,50

Obtenção de frequência

Attendance to 80% of classes is required (20% of unjustified absences) in the sum of the 2 types of classes, for access to the continuous assessment regime.

Fórmula de cálculo da classificação final

Continuous assessment:
FC = 90 % (average of 2 tests) + 10% (small project)

Exam(s):
FC = 100 % Exam OR 90% Exam + 10% (small project) – the best of the 2 options.

Provas e trabalhos especiais

The cloning project will be partially elaborated in class, as a way of contextualizing theoretical concepts and their application to solve a given problem. The evolution of the project will be monitored throughout the semester, establishing intermediate "deadlines" to complete specific stages. The scheduling of the stages to be developed in the project assumes that it is completed three weeks before the end of the semester, but will have an extended deadline for delivery until the end of the academic period.

Avaliação especial (TE, DA, ...)

There is no provision for any special assessment regime, not included in those already provided for in the Regulation.