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Biocatalysis and Bioremediation

Code:

MEBQ006

Sigla:

BB

Áreas Científicas
Classificação	Área Científica
OFICIAL	Biotecnologia

Ocorrência: 2022/2023 - 2S

Ativa?	Yes
Unidade Responsável:	Departamento de Engenharia Química e Biológica
Curso/CE Responsável:	Master in Biological and Chemical Engineering

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
MEBQ	18	Study Plan	1	-	5	45	135

Língua de trabalho

Portuguese
Obs.: Português suitable for english speaking students

Objetivos

It is intended that students acquire in-depth knowledge of biological catalysis, so that in the end of the semester they know examples of biocatalysis in non-conventional media such as organic solvents, supercritical fluids and solid-solid media. It is also important that students understand the strategies and advantages of addressing environmental contamination using microorganisms, by taking into account the concerned pollutants.

Resultados de aprendizagem e competências

Not applicable

Modo de trabalho

Presencial

Programa

1. Biocatalysis in non-conventional media (NAM). Biocatalysis in organic solvents. Stability and enzymatic selectivity in NAM. Biocatalysis in the presence of ionic liquids. Biocatalysis in supercritical fluids. Biocatalysis in solid-solid systems. Biocatalysis in solid-gas systems. Greener NAMs. Physico-chemical aspects of biocatalysis in NAM.


2. Biosensors, miniaturization of microelectrodes. Examples of biotransformations carried out on an industrial scale.


3. Technologies of bioremediation.


4. Enzymes used/improved for bioremediation.


5. Wastewater treatment with microbial fuel cells. Enzymatic conversion of waste biomass and CO₂.


6. Approach to the problem of environmental contamination by hazardous compounds. Types of pollutants (organic and inorganic) and transport mechanisms in contaminated sites.


7. Principles of biodegradation of pollutants. Microbial ecology. Factors that influence biodegradation. Mechanisms of biodegradation of pollutants.

Bibliografia Obrigatória

Cabral, J.M.S., Gama M., Aires-Barros, M.R.M., ; Engenharia Enzimática, Lidel, 2003. ISBN: 9789727572724

Bibliografia Complementar

Bommarius, A.S., Riebel-Bommarius; Biocatalysis: Fundamentals and Applications, Wiley-Blackwell, 2004. ISBN: 978-3-527-30344-1
Singh, A., Ward, O.P; Biodegradation and Bioremediation, Springer, 2004
Buchholz K,, Kasche V., Bornscheuer UT; Biocatalysts and Enzyme Technology, Wiley-Blackwell, 2004

Métodos de ensino e atividades de aprendizagem

This curricular unit comprises a theoretical / practical component, consisting of: presentations of theoretical concepts with informatical support of PowerPoint slides and an element of practical application for exercise resolution, which merges with the exposition of the theoretical contents. There is still a component of experimental classes for holding a small number of activities for better consolidation of the transmitted lectures.

The assessment by continues evaluation throught the term, presupposes the accomplishment of all the elements: the test, research work and laboratory work with report.

The classification by final exam can be 100% of the classification  or 75% the exam classification + 25% the evaluation of the report of the laboratory work.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	50,00
Trabalho escrito	25,00
Trabalho laboratorial	25,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Frequência das aulas	45,00
Estudo autónomo	60,00
Trabalho escrito	20,00
Trabalho laboratorial	10,00
Total:	135,00

Obtenção de frequência

Not applicable

Fórmula de cálculo da classificação final

Continuous evaluation

0.5 * test + 0.25 * written work + 0.25 * laboratory work report


Exam

100% exam or 75% exam + 25% of the laboratory work report