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Cell and Molecular Biology

Code:

BINF009

Sigla:

BMC

Áreas Científicas
Classificação	Área Científica
OFICIAL	Biotecnologia

Ocorrência: 2023/2024 - 2S

Ativa?	Yes
Página Web:	https://moodle.ips.pt/2324/course/view.php?id=517
Unidade Responsável:	Departamento de Engenharia Química e Biológica
Curso/CE Responsável:	Undergraduate in Bioinformatics

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
BINF	34	Study Plan	1	-	5	75	135

Língua de trabalho

Portuguese

Objetivos

The objective of this curricular unit is to increase the knowledge about the basic functional unit of all living beings – the cell, in terms of its composition, dynamic and functioning.

Resultados de aprendizagem e competências

The students should know and comprehend molecular and cellular events intrinsic to the functioning, regulation and cellular differentiation, and they should acquire the capacity to have a global perspective of the working of cells and consequently of living organism. It is intended that the students acquire knowledge and be able to apply basic experimental methodologies of molecular biology.

Modo de trabalho

Presencial

Programa

1. Introduction to the Cell. Basic constituents and evolutionary aspects. Model organisms and analysis of cell components.

2. Fundamentals of Molecular Biology
2.1. Expression of genetic information.

1. Nucleic acids – structure and function;


2. heredity and genetic code;


3. organization and evolution of genomes;


4. Sequenced genomes;


5. RNA synthesis and processing. Translation. Transcriptional and post-transcriptional regulation.

2.2 Recombinant DNA techniques.

1. Endonucleases and Ligases


2. PCR technique


3. cloning and gene expression


4. New technologies

3. Internal Organization of the Eukaryotic Cell

3.1. Nucleus: transport between nucleus and cytoplasm; nucleolus.

3.2 Endomembrane System. Protein processing and addressing. Vesicular transport. endocytosis and phagocytosis

3.3 Bioenergetics: mitochondria, chloroplasts and photosynthesis, peroxisomes.

3.4 Cellular Signaling. Membrane receptors and signal transduction pathways. Development, differentiation and apoptosis.

Bibliografia Obrigatória

coord.Carlos Azevedo, Cláudio E. Sunkel; Biologia celular e molecular, Lidel , 2012. ISBN: ISBN 978-972-757-692-0
Bruce Alberts ...[et al.]; Molecular biology of the cell, Garland Science, , 2008. ISBN: ISBN 0-8153-4106-7 (5th ed.)
David Sadava ...[et al.]; Life : the science of biology, Sinauer Associates, 2014. ISBN: ISBN 978-1-4641-3639-9 (10th ed)
T. A Brown; Gene Cloning and DNA Analysis : An Introduction, John wiley and sons ltd, 2016. ISBN: 9781119072560
Jeff Hardin, Gregory Bertoni, Lewis J. Kleinsmith; Becker's world of the cell, Pearson, 2012. ISBN: ISBN 0-321-70978-0. - ISBN 978-0-321-70978-3

Bibliografia Complementar

Harvey Lodish...[et al.]; Molecular cell biology, W.H. Freeman, cop., 2008. ISBN: ISBN 978-0-7167-6017 (6th ed)
Michael R. Green, Joseph sambrook; Molecular cloning : a laboratory manual, CSH Press, 2012. ISBN: ISBN 978-193611342-2 (4 th ed)

Métodos de ensino e atividades de aprendizagem

The curricular unit is organized into two major components, one theoretical and the other theoretical-practical, and has a small practical laboratory component. The theoretical content will be presented using computer support (powerpoint presentations, short illustrative videos). In the theoretical-practical component, practical exercises relevant to the syllabus will be carried out, such as exercises and problems, development of tasks/group work in a perspective of problem-based learning, case-studies and research, using whenever possible online platforms and molecular biology software. In the laboratory, simple procedures for illustrating basic molecular biology techniques will be performed, followed by the interpretation of results. The prior preparation of the class, the execution skills and interpretation of the results (lab notebook) will be evaluated.

Software

Benchling
Lasergene

Palavras Chave

Natural sciences > Biological sciences > Biology > Molecular biology
Natural sciences > Biological sciences > Biology > Cell biology

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Apresentação/discussão de um trabalho científico	10,00
Teste	70,00
Participação presencial	10,00
Trabalho laboratorial	10,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Apresentação/discussão de um trabalho científico	15,00
Estudo autónomo	45,00
Frequência das aulas	60,00
Trabalho laboratorial	15,00
Total:	135,00

Obtenção de frequência

In the laboratory component, only one absence is allowed in the 3 scheduled classes.
Attendance at theoretical and theoretical-practical classes is not a criterion for access to continuous assessment regime.

The contribution of the "Class Participation" and "Laboratory Component" components in the assessment by exam requires that the attendance condition be met (70% attendance).

Fórmula de cálculo da classificação final

Continuous evaluation:
NF= 70% (T1 +T2)/2 + 10% TG + 10% PA+ 10% CL
Minimum average score for the Tests is 9.0 val.

T - Test
TG - Group Work (Molecular Biology Topics)
PA - Participation in classroom activities
CL - Laboratory Component

Assessment by Exam:
NF= 100% Exam OR Exam OR 90% Exam + 10% CL OR 80% Exam + 10% CL + 10% TG OR 70% Exame + 10% CL + 10% TG + 10% PA OR 90% Exam + 10% TG

PA, TG and CL are assessment components done during continuous evaluation.

Minimum exam score is 9.0 val.

The contribution of the "Class Participation" and "Laboratory Component" components in the assessment by exam requires that the attendance condition be met (70% attendance).

Avaliação especial (TE, DA, ...)

For students with special statuses (e.g. student workers) who are unable to attend any classes, and do some of the components, their weight will be added to the Tests components, to the limit that the continuous assessment will be:
CF = 100% (Test 1 + Test 2)/2