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Biochemistry

Code:

LTB12002

Sigla:

BIOQUI

Áreas Científicas
Classificação	Área Científica
OFICIAL	Controlo e Processos

Ocorrência: 2021/2022 - 2S

Ativa?	Yes
Unidade Responsável:	Departamento de Sistemas e Informática
Curso/CE Responsável:

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
LTB	87	Plano de Estudos	1	-	6	60	162

Língua de trabalho

Portuguese

Objetivos

. Provide knowledge regarding the structure / function of biomolecules
. Provide knowledge regarding enzymatic kinetics and regulation of cellular metabolism
. Develop basic laboratory practices for the analysis of biomolecules
. To develop laboratory practices related to analytical techniques and quantitative / qualitative tests in biochemical analysis

Resultados de aprendizagem e competências

The UC has a laboratory component that allows, in accordance with UC objectives:
-the operationalization of the concepts taught;
- the development of the scientific and experimental spirit and the capacity for organization and analysis of information;
-the learning of laboratory and analytical procedures and techniques;
-the acquisition of know-how skills and teamwork.

Modo de trabalho

Presencial

Pré-requisitos (conhecimentos prévios) e co-requisitos (conhecimentos simultâneos)

Chemistry
. acid-base reactions and equilibrio
. redox reactions

Programa

Theoretical program:
1.General considerations
. water as solvent
. acids and bases
. buffer solutions
2. Biomolecules
Proteins:
. structure and function
. enzymatic kinetics
DNA and RNA:
. structure, function and protein synthesis
Carbohydrates:
. structure and function
Lipids and membranes:
. structure and transport systems
3. Bioenergetics
4. Metabolism
.Central metabolic pathway

TP component
Resolution of exercises on:
. Calculation of concentration / dilution of solutions
. Proteins
. Kinetics
. Spectrophotometric determination
. Carbohydrates
. DNA
. Lipids
. Bioenergetics and Metabolism

Program of laboratory classes:
. Preparation of solutions / dilutions
. Acid-base properties of a solution
. Properties of proteins
. Spectroscopic Characterization / Assay
. Isolation and purification of components of a mixture
. Electrophoresis (proteins and DNA)
. Identification of biomolecules

Bibliografia Obrigatória

Stryer, L.; Biochemistry, 4th Ed, W. H. Freeman and Company, New York , 1995
Manuel Júdice Halpern; Bioquímica, Lidel, 1996
Devlin, T.M.; Textbook of Biochemistry with clinical correlations, 6th Ed., Wiley-Liss, 2006

Bibliografia Complementar

Quintas, A., Freire, A.P., Halpern, M.J.; Bioquímica - organização molecular da vida, 1Ed, Lidel, 1998

Métodos de ensino e atividades de aprendizagem

In the classes the participative method is adopted, being privileged the interactive formation even in the introduction of theoretical concepts. It is intended to lead students to draw conclusions, guiding them in this process.

The UC has a laboratory component, aiming to illustrate in practice the theoretical concepts transmitted. In the laboratory classes, the students execute experimental works according to protocols supplied, elaborating later the respective questionnaire or report. In these classes the experimental and collaborative method (group work) is put into practice.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Apresentação/discussão de um trabalho científico	15,00
Teste	70,00
Trabalho laboratorial	15,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Apresentação/discussão de um trabalho científico	15,00
Trabalho escrito	70,00
Trabalho laboratorial	15,00
Total:	100,00

Obtenção de frequência

-Tests (minimum 9,5 val.) 
or
-Examination (minimum 9,5 val.)

- Mandatory laboratories (without minimum mark)

Preparation of the work, coordination of the group and (one) report. Pratical final evaluation
Note: Only 1 foul is allowed

Seminar - oral presentation on a theme of choice

Final mark = 0.7 * Tests/examination + 0.15 * laboratory + 0.15 * oral presentation

Student Worker students (with TE status recognized) who do not attend laboratory classes (or lack more than 1 laboratory work), will perform in the final exam, a specific group specifically related to the laboratory component of the CU. The approval to UC implies a positive classification in this specific group (regardless of the classification obtained in the other groups of the exam). This group will have a weight of 30% in the overall price of the exam.

TE students who choose to perform continuous assessment (tests) and who do not attend laboratory classes (or lack more than 1 laboratory work), should also make this specific group on the laboratory component of the UC, on the date of the exam Normal. The approval to the CU implies a positive classification in this specific group (regardless of the classification obtained in the tests). This group will have a weight of 30% in the overall price of the exam.

Fórmula de cálculo da classificação final

Final mark = 0.7 * Tests/examination + 0.15 * laboratory + 0.15 * oral presentation

Avaliação especial (TE, DA, ...)

Student Worker students (with TE status recognized) who do not attend laboratory classes (or lack more than 1 laboratory work), will perform in the final exam, a specific group specifically related to the laboratory component of the CU. The approval to UC implies a positive classification in this specific group (regardless of the classification obtained in the other groups of the exam). This group will have a weight of 30% in the overall price of the exam.


TE students who choose to perform continuous assessment (tests) and who do not attend laboratory classes (or lack more than 1 laboratory work), should also make this specific group on the laboratory component of the UC, on the date of the exam Normal. The approval to the CU implies a positive classification in this specific group (regardless of the classification obtained in the tests). This group will have a weight of 30% in the overall price of the exam.

Melhoria de classificação

It is only possible to perform grade improvement only at the time of Appeal

Observações

The Professor reserves the right to convene an oral presentation whenever he deems it necessary.

Students with a grade ≥ 17 will be subject to an oral