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Environmental Microbiology

Code:

LTAM08

Sigla:

MA

Áreas Científicas
Classificação	Área Científica
OFICIAL	Controlo e Processos

Ocorrência: 2021/2022 - 2S

Ativa?	Yes
Unidade Responsável:	Departamento de Sistemas e Informática
Curso/CE Responsável:	Environmental and Marine Technology

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
LTAM	49	Plano de Estudos 2016/17	1	-	6	75	162

Língua de trabalho

Portuguese

Objetivos

It is intended that students:
-Avalise and recognize the contribution of Microbiology in other sciences and their applications
-Identify the different classes of micro-organisms and their characteristics
-Recognize the main techniques for inoculation, growth and preservation of microorganisms in the laboratory as well as methods of growth control
- Can explain the diversity of habitats vs. nutritional needs and physical needs, as well as the different ways of obtaining and storing energy
-Recognise the intervention of micro-organisms in the recycling of matter and its relevance at the environmental level
At the practical level:
- Can not work under aseptic conditions
-Data skills in the manipulation of bacterial cultures for identification and isolation regardless of source (soil, water, air or other source)
- are able to determine the microbial concentration in an unknown sample using the appropriate method according to their provenance
-Able to evaluate the microbiological quality of a sample of water (residual and consumption) and air in accordance with the legislation in force

Resultados de aprendizagem e competências

The UC has a laboratory component that allows, in accordance with UC objectives:
-the operationalization of the concepts taught;
- the development of the scientific and experimental spirit and the capacity for organization and analysis of information;
-the learning of laboratory and analytical procedures and techniques;
-the acquisition of know-how skills and teamwork.

Modo de trabalho

Presencial

Programa

Theoretical

1. Introduction
- scope of study
- story
- applications
2. Biosafety
- introduction to laboratories
- in the EST laboratory
3. Microscopy techniques
- Optical vs. Electron Microscopy
- the optical microscope and the various techniques
4. Microbial evolution and diversity
- common ancestor and evolutionary scale
- classification systems
- microbial diversity
5. Fungi and Viruses
6. Cultivate in Microbiology
- aseptic technique
- culture mediums
- handling crops
- determination of [microbial]
7. Growth and control of microbial growth
- growth cycle
- direct and indirect growth measures
- physical agents and chemical agents
8. Microbiological quality of water

Laboratory

- research of microorganisms;
- optical microscopy;
- simple staining and staining of Gram;
- technique in microbiology;
- determination of the microbial concentration;
- sowing techniques;
- cultivation of bacteria and fungi;
growth control factors;
- analysis of a soil sample
- analysis of residual water;
- analysis of a sample of consumption.

Bibliografia Obrigatória

Wanda, F., Ferreira, C., De Sousa, J.C.F.; Microbiologia, 1ª Edição, LIDEL, Edições Técnicas (Mediateca), 1998
Atlas, R.M.; Principles of Microbiology 2nd Ed, WBC, Wm. C. Brown Publishers (Mediateca), 1997

Bibliografia Complementar

McKane, L., Kandel, J.; Microbiology – essentials and applications, 2nd Ed., Mc-Grall Hill, 1996
Madigan, M.T., Martinko, J.M., Parker, J.; Biology of microorganisms, 10 th Ed., Prentice Hall , 2000

Métodos de ensino e atividades de aprendizagem

The UC addresses general concepts of Microbiology and specific in the Environmental area evidenced and trained performing laboratory work with real samples.

In the classes the participative method is adopted, being privileged the interactive formation even in the introduction of theoretical concepts. It is intended to lead students to draw conclusions, guiding them in this process.
The UC has a laboratory component, aiming to illustrate in practice the theoretical concepts transmitted. In the laboratory classes, the students execute experimental works according to protocols supplied, elaborating later the respective questionnaire or report. In these classes the experimental and collaborative method (group work) is put into practice.

Tipo de avaliação

Distributed evaluation without final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	70,00
Trabalho laboratorial	30,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Apresentação/discussão de um trabalho científico	15,00
Trabalho escrito	70,00
Trabalho laboratorial	15,00
Total:	100,00

Obtenção de frequência

- Global test (minimum 9,5 val.)
or
-Examination (minimum 9,5 val.)

- Seminar (without minimum mark) - presentation of a theme of choice

- Laboratories (without minimum mark)
Preparation of the work, coordination of the group, results obtained and their interpretation (questionnaire). Pratical individual examination

Fórmula de cálculo da classificação final

Final mark = 0.7*Tests/examination + 0.3*(laboratory + oral presentation/2)

Provas e trabalhos especiais

Students can only miss one lab. If more than one laboratory is absent, they will not be able to carry out the continuous assessment.

All students can perform the missing lab work, as long as they provide justification for the absence

Worker students (with TE status recognized) will perform in the final exam, a specific group specifically related to the laboratory component of the CU.

The Professor reserves the right to call an oral examination whenever he seems it´s necessary
Students with a score of ≥ 17 will be subject to an oral

Avaliação especial (TE, DA, ...)

Student Worker students (with TE status recognized) who do not attend laboratory classes (or lack more than 1 laboratory work), will perform in the final exam, a specific group specifically related to the laboratory component of the CU. The approval to UC implies a positive classification in this specific group (regardless of the classification obtained in the other groups of the exam). This group will have a weight of 30% in the overall price of the exam.

TE students who choose to perform continuous assessment (tests) and who do not attend laboratory classes (or lack more than 1 laboratory work), should also make this specific group on the laboratory component of the UC, on the date of the exam Normal. The approval to the CU implies a positive classification in this specific group (regardless of the classification obtained in the tests). This group will have a weight of 30% in the overall price of the exam.

Melhoria de classificação

The ranking improvement can only be carried out in the appeal season