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Laboratory IVB

Code:

BIOT156

Sigla:

Lab IVB

Áreas Científicas
Classificação	Área Científica
OFICIAL	Biotecnologia
OFICIAL	Processos em Engenharia Química e Biológica

Ocorrência: 2019/2020 - 2S

Ativa?	Yes
Unidade Responsável:	Processos em Engenharia Química e Biológica
Curso/CE Responsável:	Undergraduate in Biotechnology

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
BIOT	41	Study Plan	2	-	5	60	135

Língua de trabalho

Portuguese - Suitable for English-speaking students
Obs.: Português

Objetivos

This curricular unit is based on laboratory experiences representing theoretical concepts acquired in Separation Process IB, Transport Phenomena II, and Molecular and Cellular Biology curricular units.

It is intended that, in this curricular unit, the students acquire the following competencies:

- Plan, implement, develop and optimize experiments in the area of the curricular units that the laboratory work refers.

- Interpreting results of experiments that highlight some of the basic transport phenomena, instrumental methods of analysis and biochemistry.

- Correlate the theoretical models taught, with the proper applicability in the treatment of experimental results.

- Handle material and specific equipment used in carrying out the experiments.

- Assess the importance of the accuracy of the measurements to be made.

- Writing a scientific report clearly and objectively.

Resultados de aprendizagem e competências

-

Modo de trabalho

Presencial

Programa

Laboratory IVB consists in a set of laboratory works involving the application of knowledge acquired in Separation Process IB, Transport Phenomena II, and Molecular and Cellular Biology.

1. Determination of transfer coefficients and mass transfer rate between Gas-Liquid phase, and Solid-Liquid phase.
2. Sieve analysis; Determination of settling velocity in two different fluids;
3. Determination of specific resistance of filter medium, the resistance of the cake and the coefficient of compressibility.
4. Curve of bacterial growth in liquid culture medium and counting CFU / ml.
5. Extraction of DNA. Purification of plasmidic DNA. DNA analysis using the agarose gel electrophoresis technique.

Bibliografia Obrigatória

C. Azevedo e C.E. Sunkel; Biologia Celular e Molecular, Lidel, 2005. ISBN: 9789727576920
C. J. Geankoplis; Transport Processes and Unit Operations, Pearson Education Limited, 2013. ISBN: 9781292026022

Métodos de ensino e atividades de aprendizagem

Execution of laboratory experiences related to several curricular unit programs. Each experimental work is preceded by a discussion of scientific principles and procedures of the work protocol. The students should prepare the laboratory work by writting the necessary theoretical support, experimental procedure and calculations on the individual lab notebook, (mandatory). In the following week, students should have the results, calculations and data discussion in the lab notebook. This information must be available for evaluation by the teacher when requested.

Under distance learning, classes will be held by videoconference, whenever possible, explaining the procedure of each practical work proposed in the guide or alternatively a presentation of the procedure in commented powerpoint. Students' questions will be posted on the Teams forum. Presentations will be made available on Moodle explaining the techniques used in the proposed practical works, photographs of the equipment, reagents and laboratory material necessary to carry out the work and links to youtube videos demonstrating some biotechnological laboratory techniques.

Of the total classes planned, 40% of these should be taught in person, as soon as Pandemic conditions permit, so that students can train the basic techniques used in biotechnology.

Tipo de avaliação

Distributed evaluation without final exam

Componentes de Avaliação

Designation	Peso (%)
Participação presencial	10,00
Apresentação/discussão de um trabalho científico	15,00
Teste	20,00
Trabalho escrito	10,00
Trabalho laboratorial	45,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Apresentação/discussão de um trabalho científico	5,00
Estudo autónomo	75,00
Frequência das aulas	45,00
Trabalho escrito	10,00
Total:	135,00

Obtenção de frequência

Mandatory attendance as a condition of access to continuous assessment.

Fórmula de cálculo da classificação final

Individual quizzes (6) by moodle or teams (20%); Construction of an individual laboratory notebook (20%); preparation of 1 complete report (15%), 2 simple reports (10% each) and a poster (15%) of technical and scientific nature, made in group to be submitted in moodle with discussion or oral presentation by videoconference. Performance in classes (6 classes) done at the laboratory (10%), as soon as the conditions of reopening allow it, so that students can train the basic techniques used in biotechnology.