Microbiology and Microbial Analysis

Code:

TLQB004

Sigla:

MAM

Áreas Científicas
Classificação	Área Científica
CNAEF	Biology and Biochemistry

Ocorrência: 2019/2020 - 1S

Ativa?	Yes
Unidade Responsável:	Biologia
Curso/CE Responsável:	Professional Higher Technical Course in Chemical and Biological Laboratory Technologies

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
TLQB	39	Study Plan	1	-	8	82,5	216

Docência - Responsabilidades

Docente	Responsabilidade
Sónia Alexandra Paiva dos Santos

Docência - Horas

Theorethical and Practical :

5,50

Type	Docente	Turmas	Horas
Theorethical and Practical	Totais	2	11,00
	Gabriela de Lurdes Martins da Silva		5,00
	Pedro Boto Pereira Franco Pinheiro		3,00

Língua de trabalho

Portuguese

Objetivos

At the end of the course, students are expected to understand and know the theoretical foundations: microorganisms and their diversity; the kinetics and energy of bacterial growth and cell death; the effect of environmental factors and anti-microbial agents in the control of microbial growth; the basic mechanisms underlying the adaptability and proliferation of microorganisms in different habitats. It should also learn theoretical and practical knowledge about the different types of microbial analysis, applicable standards depending on the type of sample and/or analysis. The student must also be able to apply, in the laboratory context, the knowledge
previous ones, knowing how to work safely and following the rules of asepsis, knowing how to prepare the culture media appropriate to each type of microorganism, to know methods of sterilization, disinfection and decontamination, to carry out microscopic examinations, perform sample collection and preparation, master sample dilution techniques and
innoculation, know microbial identification methods in food, water and air.

Resultados de aprendizagem e competências

Modo de trabalho

Presencial

Programa

Theoretical contents:
1. Brief history of Microbiology.
2. Diversity in the microbial world.
3. Prokaryotic cells versus eukaryotic cells.
4. Microbial morphology.
5. Microbial growth.
6. Microbial taxonomy.
7. Microbial metabolism.
8. Microbial genetic organization.
9. Microbial habitats.
10. Interactions with Man.

Laboratory Contents:
1. General safety rules in microbiology laboratories
2. Facilities, equipment and materials.
3. Methods of sterilization, disinfection and decontamination.
4. Asepsis rules.
5. Preparation of culture media.
6. Microscopic exams.
7. Collection and preparation of samples.
8. Basic rules in microbiology, dilutions and sowing.
9. Microbial identification systems.
10. Microbiological analysis of food.
11. Microbiological analysis of waters.
12. Air tests and analysis of the effectiveness of detergents and disinfectants.

Bibliografia Obrigatória

Madigan, M. et al.; Brock Biology of Microorganisms Global Edition
Prescott; Laboratory Exercises in Microbiology, McGraw Hill
Seiler; Good Laboratory Practice, Springer
OMS; Manual of basic techniques for a health laboratory
Tortola, G. J. , Funke, R. J. and Case, C. L; Microbiologia, Artemed, London
Benson; Microbiological Applications Lab Manual, McGraw Hill; Harley

Métodos de ensino e atividades de aprendizagem

In theoretical classes, expository and interrogative techniques are used, stimulating students' reasoning and critical spirit through individual work. In laboratory classes, students carry out experimental work to train microbial analysis techniques and make reports to develop critical thinking, debate and group work.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	50,00
Apresentação/discussão de um trabalho científico	5,00
Trabalho escrito	45,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	83,50
Frequência das aulas	82,50
Trabalho escrito	49,00
Apresentação/discussão de um trabalho científico	1,00
Total:	216,00

Obtenção de frequência

Mandatory attendance - 80%

Fórmula de cálculo da classificação final

Evaluation in the academic period:

25% Test 1 + 25% Test 2 + 10% Work 1 + 10% Work 2 + 10% Work 3 + 10% Work 4 + 10% Work 5.

Exam (all seasons):

50% Exam + 10% Work 1 + 10% Work 2 + 10% Work 3 + 10% Work 4 + 10% Work 5.

Works 1 to 4 are reports of practical activities carried out, in groups of 3 to 4 elements, in practical laboratory classes.

- Work 5 is a work of bibliographic research on various topics, related to the various study areas of Microbiology, bacteriology, virology, mycology, parasitology and algology, carried out in groups and with oral presentation (the weight for oral presentation / discussion is 50%). Students in the same group may be given a different classification based on the quality of the oral presentation and the mastery of the topic.

- The submission of Works 1 to 5 is mandatory to obtain approval.

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