Cell and Tissue Technology
Áreas Científicas |
Classificação |
Área Científica |
OFICIAL |
Biotecnologia |
Ocorrência: 2019/2020 - 2S
Ciclos de Estudo/Cursos
Sigla |
Nº de Estudantes |
Plano de Estudos |
Anos Curriculares |
Créditos UCN |
Créditos ECTS |
Horas de Contacto |
Horas Totais |
MEBQ |
18 |
Study Plan |
1 |
- |
5 |
52,5 |
135 |
Docência - Responsabilidades
Língua de trabalho
Portuguese
Objetivos
Provide students with an integrated education of cell and tissue technology, based on fundamental concepts of Cellular and Molecular Biology, Genetics, Immunology and bio-process, with applications in human and veterinary medicine.
Resultados de aprendizagem e competências
Not Applicable
Modo de trabalho
À distância
Programa
- Introduction to Animal and Tissue technology: history; differences between animal cells and microorganisms; examples of animal cells applications in commercial production
- Basics concepts on Animal Cells: typical structure of animal cells; type of cellular cultures; cellular growth and propagation; cryopreservation; storage; quality control and safety; principal cell lines used in the industry; insect cells
- Cloning and heterologous expression in animal cells: genetic flow of information and molecular cloning; elements that are needed for the genetic expression in eukaryotic cells; heterologous expression systems in animal cells; viral DNA vectors; viral RNA vectors; baculovirus; plasmidic vectors; cells lines in biotechnological processes; expression in animal cells; DNA transfection in animal cells; selection markers
- Cell Metabolism and its control: energy sources; metabolic bioproducts; factors affecting the cellular metabolism
- Post-translational modification of recombinant proteins
- Mechanisms of cells proliferation and death
- Culture media for animal cells
- Bioreactors for animal cells: bioreactors project and operation; cellular kinetics; modelling; monitorization and control of cell cultures
- Animal cells separation and purification methods
- Cells therapy and stem cells
- Gene therapy: case study – adenoviral vectors production (for vaccination and gene therapy)
- Tissue Microfabrication.
Bibliografia Obrigatória
Leda R. Castilho, ângela Maria Moraes, Elisabeth F.P. Augusto and Michael Butler; Animal Cell Technology: From Biopharmaceuticals to Gene Therapy
R. Ian Freshney; Culture of Animal Cells
Clemens van Blitterswijk, Peter Thomsen, Anders Lindahl, Jeffrey Hubbell, David Williams, Ranieri Cancedda, Joost de Bruijn, Jerôme Sohier; Tissue Engineering
Palsson, B.Ø., Bhatia, S.N., ; Tissue Engineering
C. van Blitterswijk; Tissue Engineering
Doyle, A., Griffiths, J.B.; Cell and Tissue Culture
Lanza, R., Langer, R. e Vacanti, J.; Principles of Tissue Engineering
Koller, M., Palsson, B. e Masters, J.; Human Cell Culture, Vol. IV, Primary Hematopoietic Cells
Marshak, D., Gardner, R., Gottlieb, D.; Stem Cell Biology
Atala, A., Lanza, R., Thomson, J., Nerem, R.; Principles of Regenerative Medicine
Métodos de ensino e atividades de aprendizagem
For the Academic year 2019-2020, the classes were performed remotely
Tipo de avaliação
Distributed evaluation with final exam
Componentes de Avaliação
Designation |
Peso (%) |
Apresentação/discussão de um trabalho científico |
100,00 |
Total: |
100,00 |
Componentes de Ocupação
Designation |
Tempo (Horas) |
Frequência das aulas |
52,50 |
Estudo autónomo |
82,50 |
Total: |
135,00 |
Obtenção de frequência
Not Aplicable
Fórmula de cálculo da classificação final
Continuous evaluation:
NF= 50% Work 1+ 50% Work 2
Exam (all phases):
NF=100% Exam