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Molecular Genetics Techniques

Code:

TLQB017

Sigla:

TGM

Áreas Científicas
Classificação	Área Científica
CNAEF	Biology and Biochemistry

Ocorrência: 2020/2021 - 1S

Ativa?	Yes
Unidade Responsável:	Biotecnologia
Curso/CE Responsável:	Professional Higher Technical Course in Chemical and Biological Laboratory Technologies

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
TLQB	33	Study Plan	2	-	6	60	162

Língua de trabalho

Portuguese

Objetivos

At the end of this course it is intended that the student:
- Understand the role of genes as the molecular basis of the mechanisms of genetic alteration and its fundamental importance for the establishment of recombinant DNA technology;
- master Molecular Biology and Genetic Engineering methodologies that are the basis for genetic analysis and genetic research at the molecular level, namely identification of serotypes, genotypes, etc.;
- to apply recombinant DNA techniques for cloning in microrganisms.

Resultados de aprendizagem e competências

.

Modo de trabalho

Presencial

Programa

Theoretical component:
1. Introduction to Genetic Engineering.
2. Recombinant DNA technology, role of restriction enzymes and vectors.
3. Nucleic acid visualization and quantification methods: agarose gel electrophoresis, Southern and Northern blot.
4. Cloning of genes.
5. Expression systems.
6. DNA libraries.
7. Amplification of nucleic acids by PCR (qualitative and quantitative techniques: qPCR, RT-PCR, Real Time-PCR).
8. Sequencing Methods
9. gene therapy

Laboratory Application Component:
1. Nucleic acid extraction (genomic DNA, plasmid DNA and RNA)
2. Analytical and quantitative agarose gel electrophoresis;
3. Digestion of DNA with specific enzymes – restriction pattern;
4. PCR amplification of DNA samples;
5. Cloning of genes in expression vectors;
6. Recovery of DNA samples from agarose gel.

Bibliografia Obrigatória

T. A. Brown; Gene Cloning and DNA Analysis : An Introduction, Wiley Blackwell, , 2016. ISBN: 978-1-119-07256-0
Desmond S. T. Nicholl; An introduction to genetic engineering, Cambridge University Press, 2008. ISBN: 978-0-521-61521-1
Michael R. Green, Joseph Sambrook; Molecular cloning : a laboratory manual, CSH Press, 2012. ISBN: 978-193611342-2
Keith Wilson, John Walker; Principles and techniques of biochemistry and molecular biology, Cambridge University Press, 2010. ISBN: 978-0-521-73167-6
A. J. Nair; Introduction to biotechnology and genetic engineering, Jones & Bartlett Learning, 2008. ISBN: 978-0-7637-7375-5
Benjamin Lewin; Genes VII, John Wiley & Sons, 2000

Métodos de ensino e atividades de aprendizagem

The contents of the UC are presented in lectures, in expository mode interspersed with examples, using the support of powerpoint slides and in the current academic year, in online mode through the Zoom platform.

The laboratory component allows the practical illustration of theoretical concepts. In laboratory classes, 3 large works are performed, covering more than 1 class/week, favoring the strategy of combining the illustration and execution of the various techniques described in the syllabus, for a specific objective.
Students are integrated into an interdisciplinary research project that involves other Graduate courses. The products (purified genetic material, and/or prepared with modifications) obtained in their work are used as raw material by the other students, who produce other recombinant genetic material which in turn serves as a sample to be analyzed in one of the last laboratory works. Works are developed that illustrate the most relevant laboratory techniques and the interpretation of results and problem solving. In the current academic year, laboratory classes were concentrated in modules of 4 to 6 hours, and interspersed with distance classes for analysis of results.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	50,00
Trabalho laboratorial	50,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	45,00
Frequência das aulas	60,00
Trabalho de investigação	12,00
Trabalho escrito	30,00
Trabalho laboratorial	15,00
Total:	162,00

Obtenção de frequência

Minimum attendance of 80% of the laboratorial classes.

Fórmula de cálculo da classificação final

Continuous Assessment:

FG = 50 % (theoretical component: 2 tests) + 50% (Laboratorial component)

Assessment by Exam*:

FG = 50% Exam + 50% (Laboratorial component)

*SPECIAL REGIME – ONLY THE THEORETICAL COMPONENT CAN BE EVALUATED BY FINAL EXAM.

Provas e trabalhos especiais

The laboratory component is evaluated by 3 parameters:

1. Laboratory work reports: 40%


2. Preparation, behavior and participation in laboratory execution: 50%


3. Preparation and maintenance of laboratory notebook: 10%.

An oral evaluation / discussion of the reports will be carried out.

Avaliação especial (TE, DA, ...)

There is no provision for any special assessment regime, not included in those already provided for in the Regulation.

For students who either by special statutes or caused by the pandemic context cannot attend some of the laboratory classes, a special regime will be created as long as >50% of the classes were atended.