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Enzymatic Engineering

Code:

MEBQ001

Sigla:

EE

Áreas Científicas
Classificação	Área Científica
OFICIAL	Biotecnologia

Ocorrência: 2022/2023 - 1S

Ativa?	Yes
Unidade Responsável:	Departamento de Engenharia Química e Biológica
Curso/CE Responsável:	Master in Biological and Chemical Engineering

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
MEBQ	19	Study Plan	1	-	6	52,5	162

Língua de trabalho

Portuguese

Objetivos

It is intended that students acquire skills in the engineering area applied to biological processes with enzymes. Thus, it is expected that at the end of the semester, students are able to choose and understand the mechanisms involved in enzyme catalysis and perform techniques for random and directed mutagenesis as well as for protein modification. In this curricular unit it is also expected that students learn the methodologies employed in the production of enzymes in bioreactors and downstream purification.

Resultados de aprendizagem e competências

.

Modo de trabalho

Presencial

Programa

- Applications of enzymes: General properties of enzymes; Classification and nomenclature of enzymes; Structure and function of enzymes;
- Protein engineering: "protein design". Directed and random mutagenesis. Molecular evolution.
- Mechanisms of enzyme catalysis; Kinetics of free and immobilized enzymes; Reactions with one or more substrates; Inhibition of enzyme activity. Effects of immobilization on the kinetics and properties of enzymes; stability and "folding" of proteins.
- Enzyme kinetics in multiphase systems. Steric and conformational effects. Partition effects. Mass transfer effect. Unconventional multiphase systems.
- Production of enzymes. Strategy for obtaining and purification of intracellular and extracellular enzymes.
- Immobilization of enzymes. Immobilization methods.
- Enzymatic phase and multiphase reactors: types and classification of enzyme reactors. Modeling of ideal and non-ideal enzymatic reactors; enzyme deactivation.

Bibliografia Obrigatória

Cabral, J.M.S., Gama M., Aires-Barros, M.R.M.; Engenharia Enzimática, Lidel, , 2003. ISBN: 9789727572724
Teixeira, J.A., Fonseca, M.M. ; Reatores Biológicos, fundamentos e aplicações, Lidel, 2006. ISBN: 9789727573660
Buchholz, K., Kasche, V., Bornscheuer, U.T.; Biocatalysts and Enzyme Technology, John Wiley & Sons, 2012. ISBN: 978-3-527-32989-2
Samuelson, J.C.; Enzyme Engineering Methods and Protocols Series: Methods on Molecular Biology, Humana Press, 2013. ISBN: 978-1-62703-292-6

Bibliografia Complementar

Lutz, S., Bornscheuer, U.T.; Protein Engineering Handbook, John Wiley & Sons, 2012. ISBN: 978-3-527-66701-7

Métodos de ensino e atividades de aprendizagem

This curricular unit comprises a theoretical/practical component. Theoretical contents are taught via PowerPoint presentations. Intercalating theory with practical applications focusing on problem solving will be done. There is still a component of experimental classes for holding a small number of activities for better knowledge consolidation.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	60,00
Trabalho escrito	10,00
Trabalho laboratorial	30,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	59,50
Frequência das aulas	52,50
Trabalho escrito	10,00
Trabalho laboratorial	40,00
Total:	162,00

Obtenção de frequência

Mandatory presence in 75% of the Practical Laboratory Classes.

Fórmula de cálculo da classificação final

Evaluation in the academic period:

Final Classification = 60% Test + 10% Work1 + 30% Work2 (Practical Lessons Report)

Work 2 is a report of practical activities carried out in practical laboratory classes.
The submission of Work 2 is mandatory to obtain approval.

Exam (all seasons):

100% Exam

ou

70% Exam + 30% Work2