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Cell and Tissue Technology

Code:

MEBQB10

Sigla:

TCT

Áreas Científicas
Classificação	Área Científica
OFICIAL	Biotecnologia

Ocorrência: 2022/2023 - 2S

Ativa?	Yes
Unidade Responsável:	Departamento de Engenharia Química e Biológica
Curso/CE Responsável:	Master in Biological and Chemical Engineering

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
MEBQ	1	Study Plan	1	-	5	52,5	135

Língua de trabalho

Portuguese

Objetivos

Provide students with an integrated education of cell and tissue technology, based on fundamental concepts of Cellular and Molecular Biology, Genetics, Immunology and bio-process, with applications in human and veterinary medicine.

Resultados de aprendizagem e competências

Not Applicable

Modo de trabalho

Presencial

Programa

1 - Introduction to Animal and Tissue technology: history; differences between animal cells and microorganisms; examples of animal cells applications in commercial production

2 - Basics concepts on Animal Cells: typical structure of animal cells; type of cellular cultures; cellular growth and propagation; cryopreservation; storage; quality control and safety; principal cell lines used in the industry; insect cells

3 - Cloning and heterologous expression in animal cells: genetic flow of information and molecular cloning; elements that are needed for the genetic expression in eukaryotic cells; heterologous expression systems in animal cells; viral DNA vectors; viral RNA vectors; baculovirus; plasmidic vectors; cells lines in biotechnological processes; expression in animal cells; DNA transfection in animal cells; selection markers

4 - Cell Metabolism and its control: energy sources; metabolic bioproducts; factors affecting the cellular metabolism

5 - Post-translational modification of recombinant proteins

6 - Mechanisms of cells proliferation and death

7 - Culture media for animal cells

8 - Bioreactors for animal cells: bioreactors project and operation; cellular kinetics; modelling; monitorization and control of cell cultures

9 - Animal cells separation and purification methods

10 - Cells therapy and stem cells

11 - Gene therapy: case study – adenoviral vectors production (for vaccination and gene therapy)

12 - Tissue Microfabrication.

Bibliografia Obrigatória

Leda R. Castilho, ângela Maria Moraes, Elisabeth F.P. Augusto and Michael Butler; Animal Cell Technology: From Biopharmaceuticals to Gene Therapy
R. Ian Freshney; Culture of Animal Cells
Clemens van Blitterswijk, Peter Thomsen, Anders Lindahl, Jeffrey Hubbell, David Williams, Ranieri Cancedda, Joost de Bruijn, Jerôme Sohier; Tissue Engineering
Palsson, B.Ø., Bhatia, S.N., ; Tissue Engineering
C. van Blitterswijk; Tissue Engineering
Doyle, A., Griffiths, J.B.; Cell and Tissue Culture
Lanza, R., Langer, R. e Vacanti, J.; Principles of Tissue Engineering
Koller, M., Palsson, B. e Masters, J.; Human Cell Culture, Vol. IV, Primary Hematopoietic Cells
Marshak, D., Gardner, R., Gottlieb, D.; Stem Cell Biology
Atala, A., Lanza, R., Thomson, J., Nerem, R.; Principles of Regenerative Medicine

Métodos de ensino e atividades de aprendizagem

The teaching methodologies rely on a dynamic basis of theoretical/practical classes wherein lectures will be given to present the concepts regarding the syllabus, via PowerPoint slides. Practical applications will intercalate, in which case studies will be presented, analysed, studied and discussed, by the students under the supervision of the teacher.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Apresentação/discussão de um trabalho científico	50,00
Teste	50,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	82,50
Frequência das aulas	52,50
Total:	135,00

Obtenção de frequência

Not Applicable

Fórmula de cálculo da classificação final

Continuous evaluation:

If evaluation is performed remotely:
Final Grade= 50% Work 1+ 50% Work 2 (oral presentation)

or

If evaluation is performed presentially:
Test = 50% test 1 + 50% test 2 


Exam (all seasons):

Final Grade=100% Exam (can be performed remotely or presentially. If performed remotely, an oral exame will be performed)