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Advanced Genetic Engineering

Code:

MEBQB02

Sigla:

EGA

Áreas Científicas
Classificação	Área Científica
OFICIAL	Biotecnologia

Ocorrência: 2023/2024 - 1S

Ativa?	Yes
Unidade Responsável:	Departamento de Engenharia Química e Biológica
Curso/CE Responsável:	Master in Biological and Chemical Engineering

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
MEBQ	0	Study Plan	1	-	6	52,5	162

Língua de trabalho

Portuguese

Objetivos

It is intended that students acquire skills in genetic engineering for the production of recombinant proteins in prokaryotes and eukaryotes, using several types of cloning strategies and in silico analysis. It is also intended that students develop knowledge that allow the update and progress in the area of scientific application of Molecular Biotechnology.

Resultados de aprendizagem e competências

Not applicable.

Modo de trabalho

Presencial

Programa

-  Strategies for improving production of recombinant proteins. Criteria for the choice of an expression system of heterologous proteins in bacteria, and using the pET and PQE vectors. Expression systems in eukaryotes: yeast (Picchia pastoris); insect (baculovirus); mammals; viral vectors for expression in mammalian cells; promoters used in the construction of vectors for mammalian cells; for plants with Agrobacterium.

-  Methodologies to promote genetic fusion and genetic interruption: "one step disruption method" and "PCR-generated short and long flanking homology". Primer design for fusion, disruption, directed mutagenesis and gap repair. Cloning strategies. Gene regulation by RNA Interference.

-  In silico analysis of sequences. Nucleotidic sequence searches. Alignment of DNA or protein sequences. In silico analysis of the topology of a protein. Phylogenetic analysis of proteins.

- Fundamentals of microbial evolution.

Bibliografia Obrigatória

Jeremy W. Dale, Simon F. Park; Molecular Genetics of Bacteria, Wiley Blackwell, 5.ª Edição, 2010
T.A. Brown; Gene Cloning and DNA Analysis: An Introduction, Wiley Blackwell, 6.ª Edição, 2010
G. Gibson, S.V. Muse; A Primer of Genome Science, Sinauer Associates, Inc., 3.ª Edição, 2008
Carlos A. Rosa, Gábor Péter ; The Yeast Handbook – Biodiversity and Ecophysiology of Yeast, Springer Verlag, 2006
Jocelyn E. Krebs, Benjamin Lewin, Elliott S. Goldstein, Stephen T. Kilpatrick; Lewin's Essential Genes, Jones and Bartlett, 2.ª Edição, 2009
S.B. Primrose e R.M. Twyman; Principles of Gene Manipulation and Genomics, Blackwell Publishing, 8.ª Edição, 2011
A.D. Baxevanis, B.F.F. Ouellette; Bioinformatics: a practical guide to the analysis of genes and proteins, John Wiley & Sons, 2005
B.G. Hall; Phylogenetic Trees Made Easy: a How-to Manual, Sinauer Associates, 4.ª Edição, 2011

Métodos de ensino e atividades de aprendizagem

This curricular unit comprises a theoretical/practical component. The theoretical contents are exposed via a computer readable presentations of PowerPoint and are accompanied by exercises of practical application that includes classes for the analysis of case studies and mini-projects that can be partially tested in the laboratory, in the Practical Laboratorial component of the CU.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	80,00
Apresentação/discussão de um trabalho científico	10,00
Trabalho laboratorial	10,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	49,50
Frequência das aulas	52,50
Trabalho de investigação	30,00
Trabalho escrito	30,00
Total:	162,00

Obtenção de frequência

Not applicable.

Fórmula de cálculo da classificação final

(CE): FG=40% Test 1 + 40% Test 2 + 10% Report/mini-project + 10% Presentation/Discussion of a scientific work
or
(E): FG=100% Exam ou 80% Exam + 10% Report/mini-project + 10% Presentation/Discussion of a scientific work

Provas e trabalhos especiais

Assignment 1 - Elaboration of a Report/mini-project - Report of a laboratory class or elaboration of a protocol on a possible experimental work.
Assignment 2 - Presentation in English and oral discussion of a research work

Observações

The assignments can be developed individually or in groups of 2 to 3 members.