Microbiology and Microbial Analysis

Code:

TLQE004

Sigla:

MAM

Áreas Científicas
Classificação	Área Científica
CNAEF	Biology and Biochemistry

Ocorrência: 2023/2024 - 2S

Ativa?	Yes
Página Web:	https://moodle.ips.pt/2324/course/view.php?id=2417
Unidade Responsável:	Departamento de Engenharia Química e Biológica
Curso/CE Responsável:

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
TLQBE	16	Study Plan	1	-	6	67,5	162

Docência - Responsabilidades

Docente	Responsabilidade
Marta Sofia Guedes de Campos Justino

Docência - Horas

Theorethical and Practical :	2,00
Practical and Laboratory:	2,50

Type	Docente	Turmas	Horas
Theorethical and Practical	Totais	1	2,00
Theorethical and Practical	Susana Isabel Nunes Magriço		2,00
Practical and Laboratory	Totais	1	2,50
Practical and Laboratory	Susana Isabel Nunes Magriço		2,50

Língua de trabalho

Portuguese

Objetivos

At the end of the course, students are expected to understand and know the theoretical foundations: microorganisms and their diversity; the kinetics and energy of bacterial growth and cell death; the effect of environmental factors and anti-microbial agents in the control of microbial growth; the basic mechanisms underlying the adaptability and proliferation of microorganisms in different habitats. It should also learn theoretical and practical knowledge about the different types of microbial analysis, applicable standards depending on the type of sample and/or analysis. The student must also be able to apply, in the laboratory context, the knowledge
previous ones, knowing how to work safely and following the rules of asepsis, knowing how to prepare the culture media appropriate to each type of microorganism, to know methods of sterilization, disinfection and decontamination, to carry out microscopic examinations, perform sample collection and preparation, master sample dilution techniques and
innoculation, know microbial identification methods in food, water and air.

Resultados de aprendizagem e competências

Modo de trabalho

Presencial

Programa

Theoretical contents:
1. Brief history of Microbiology.
2. Diversity in the microbial world.
3. Microbial morphology.
4. Microbial growth.
5. Microbial taxonomy.
6. Microbial metabolism.
7. Microbial habitats.
8. Food microbiology.
9. Microbiology of water for human consumption
10. Microbial interactions with Man.

Laboratory Contents:
1. General safety rules in microbiology laboratories
2. Facilities, equipment and materials.
3. Methods of sterilization, disinfection and decontamination.
4. Asepsis rules.
5. Preparation of culture media.
6. Microscopic exams.
7. Collection and preparation of samples.
8. Basic rules in microbiology, dilutions and sowing.
9. Microbial identification systems.
10. Microbiological analysis of food.
11. Microbiological analysis of waters.
12. Air tests and analysis of the effectiveness of detergents and disinfectants.

Bibliografia Obrigatória

Madigan, M. et al.; Brock Biology of Microorganisms Global Edition
Prescott; Laboratory Exercises in Microbiology, McGraw Hill
Seiler; Good Laboratory Practice, Springer
OMS; Manual of basic techniques for a health laboratory
Tortola, G. J. , Funke, R. J. and Case, C. L; Microbiologia, Artemed, London
Benson; Microbiological Applications Lab Manual, McGraw Hill; Harley

Métodos de ensino e atividades de aprendizagem

In theoretical classes, expository and interrogative techniques are used, stimulating students' reasoning and critical spirit through individual work. In laboratory classes, students carry out experimental work to train microbial analysis techniques and make reports to develop critical thinking, debate and group work.

Palavras Chave

Natural sciences > Biological sciences > Biology > Microbiology
Natural sciences > Biological sciences > Biodiversity
Natural sciences > Agrarian Sciences > Zootechnics > Feed contamination
Natural sciences > Agrarian Sciences > Soil science > Soil biodiversity
Technological sciences > Technology > Laboratory technology

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	50,00
Trabalho escrito	40,00
Trabalho laboratorial	10,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	47,00
Frequência das aulas	67,50
Trabalho escrito	46,50
Apresentação/discussão de um trabalho científico	1,00
Total:	162,00

Obtenção de frequência

Mandatory attendance - 80%

Fórmula de cálculo da classificação final

Continuous Evaluation :
CF = 50% theoretical component (CT) + 50% practical component (CP)

CT = 25% Test 1 + 25% Test 2
Minimum average test score is 9.5 val.

CP = 20% Work1 + 15% Work2 + 15% Work3
Minimum grade for the practical component (CP) is 9.5 val.

Work 1: group report
Work 2: group poster
Work 3: individual provision in the laboratory

Evaluation by Exam:
UC with Exception Regime - 100% exam not applicable: Only the theoretical component can be assessed by exam.
CF= 50% Exam + 50% CP
Minimum exam score is 9.5 val.

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