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Environmental Microbiology

Code:

LTAM08

Sigla:

MA

Áreas Científicas
Classificação	Área Científica
OFICIAL	Controlo e Processos

Ocorrência: 2022/2023 - 2S

Ativa?	Yes
Unidade Responsável:	Departamento de Sistemas e Informática
Curso/CE Responsável:	Environmental and Marine Technology

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
LTAM	49	Plano de Estudos 2016/17	1	-	6	75	162

Língua de trabalho

Portuguese

Objetivos

The objectives of the curricular unit (UC) are:

- Know the fundamental concepts of general microbiology, and the cultivation techniques and identification in microbiology.

- Know the characteristics of the different microbial classes and infectious agents. Understand the interactions between microorganisms, the living organisms, and the environment. Understand the important role of microorganisms in the balance of ecosystems. Recognize the importance of microbial ecology in environmental microbiology.

- Know the main techniques of inoculation, growth, and preservation of microorganisms in the laboratory. Know the different methods of growth control.

- Recognize the microorganisms as agents in water treatment processes, waste treatment and bioremediation.

- Know the main pathogenic microorganisms present in water, air, and organic fertilizers.

- Recognize some microorganisms as indicators of microbiological quality of water, indoor air, and organic fertilizers

- Know, understand, and search in the current legislation, the reference analytical methods to evaluate the quality of indoor air, water, and organic fertilizers.

- Use reference analytical methods to determine microbiological parameters to assess the legal compliance of indoor air, water, and organic fertilizers.

- Know and use biosafety practices in microbiological analysis laboratories.

Resultados de aprendizagem e competências

The student acquires:

- Knowledge in the area of general, environmental, and food microbiology in accordance with the objectives of the UC (see details in the UC program);

- Laboratory knowledge in the basic techniques of general microbiology, in the reference analytical methods to determine the microbiological parameters to assess the quality of water and food, and in the organizational skills (teamwork) and analysis of experimental data.

Modo de trabalho

Presencial

Programa

Theoretical Component:

- Scope of the Curricular Unit, Biosafety, and Applications.

- Brief history of microbiology.

- Concepts of microscopic; optical microscopy and staining techniques; electron microscopy; microbial taxonomy and phylogeny; binomial nomenclature; morphological and ultrastructural characteristics of prokaryotic and eukaryotic microorganisms; main microbial groups (bacteria, archaea, fungi, algae, protozoa) and infectious agents (viruses, and prions).

- Microbial growth; culture media; isolation techniques; growth curves; methods for quantitative assessment of bacterial growth; intrinsic and extrinsic factors that influence microbial growth.

- Brief history of environmental microbiology and main microorganisms present in environmental and food samples.

- Main microorganisms present in the soil and their importance in nutrient cycling and plant nutrition; beneficial and pathogenic microorganisms for plants; microbiological control of organic fertilizers.

- Microbiological quality control of indoor air; sampling and cultivation methods; current legislation for indoor air quality; microbiological parameters for indoor air.

- Microbiological quality control of water; water treatment methods; reference analytical methods and confirmatory tests; current legislation for water quality; microbiological parameters for each type of water.

Laboratory Component:

- Introduction to the laboratory of microbiology; preparation and sterilization of material; preparation and sterilization of culture media; training on basic equipment of the microbiology laboratory.

- Sampling and cultivation of microorganisms from different environments.

- Identification of different types of microorganisms with environmental relevance using microscopic techniques.

- Method of serial dilution.

- Effect of physical and chemical factors on the growth of microorganisms and determination of the conditions to control bacterial growth.

- Execution of several reference analytical methods to determine the microbiological parameters of water, air, surfaces, and organic fertilizers, and assessment of compliance with legal and regulatory specifications (i.e., sampling, techniques to determine the microbiological parameters and confirmatory tests).

 

Bibliografia Obrigatória

M.T. Madigan, J.M. Martinko, J. Parker; Brock biology of microorganisms; 10th Ed, Prentice Hall, 2003
M.T. Madigan, J.M. Martinko, K.S. Bender, D.H. Buckley, D.A. Stahl; Microbiologia de Brock, 14th Ed, artmed, 2016

Bibliografia Complementar

R.M. Atlas; Principles of Microbiology, 2nd Ed, WBC, Wm. C. Brown Publishers, 1997
L. McKane, J. Kandel; Microbiology – Essentials and Applications, 2nd Ed, Mc-Grall Hill, 1996
I.L. Pepper, C.P. Gerba, T.J. Gentry; Environmental microbiology; 3nd Ed., Academic Press, 2015

Métodos de ensino e atividades de aprendizagem

The UC is divided into a theoretical component and a laboratory component. In the theoretical classes, the participatory-expository method is adopted, even in the introduction of theoretical concepts. The aim is to lead the students to draw conclusions, guiding them in this process. In the laboratory classes, the experimental and collaborative method (teamwork) is used. In these classes, it is intended to illustrate, in practice, the theoretical concepts. The students carry out experimental works according to the given protocols and then write the respective reports.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	70,00
Trabalho laboratorial	30,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	87,00
Frequência das aulas	30,00
Trabalho laboratorial	45,00
Total:	162,00

Obtenção de frequência

THEORETICAL MARK
Approval in the theoretical component can be obtained by continuous evaluation (2 tests). The arithmetic average of the tests must be higher or equal to 9.5 values (attention: the mark of each test must be higher or equal to 7.5 values). The student can obtain approval in the theoretical component by exam. 

LABORATORY MARK
Approval in the laboratory component is obtained with a mark higher or equal to 9.5 values.

 FINAL MARK
The final mark is the weighted average of the theoretical mark (70%) and the laboratory mark (30%).

 - Evaluation of theoretical classes can be obtained by continuous evaluation but requires a minimum class attendance of 70%.

- Evaluation of laboratory classes require the attendance at the laboratory classes, delivery the respective reports and the final discussion of the reports. A MAXIMUM of 1 missed laboratory class is allowed.

- The students with Worker-Student status, who miss more than 1 laboratory class, will obligatorily perform a group of questions specifically related to the laboratory component in the final exam. The Worker-Students that choose to carry out continuous assessment and that miss more than 1 laboratory class must also perform this group of questions specifically related to the laboratory component, on the date of the Normal Season Exam.

Fórmula de cálculo da classificação final

Final mark = Test nº 1 mark x 0,35 + Test nº 2 mark x 0,35 + Laboratory mark x 0,30

 

Avaliação especial (TE, DA, ...)

- Working Students, High-Competition Athletes, Associative Directors, and Students under the Religious Freedom Law must send an email to the Professor (nuno.borges@estsetubal.ips.pt), until the second week of the semester, to present their relevant specificities, in the terms stated in the respective diplomas.

Melhoria de classificação

- The student may improve his/her classification during the Appeal Season, and for that purpose he/she must register in the Academic Division.

Observações

- Clarification of doubts: The student should express his/her intention by email (nuno.borges@estsetubal.ips.pt). Subsequently, a clarification session will be scheduled at office F105.
- Support materials are available on Moodle 2022/2023.
- The use of mobile phones is prohibited during classes and evaluation moments.
- The students can consult the evaluation tests (test or exam). For that, they should send an email to the Professor (nuno.borges@estsetubal.ips.pt) until one week before the next evaluation moment, expressing the intention to exercise this right.