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Genetic and Genotoxicity

Code:

LTB31002

Sigla:

GENG

Áreas Científicas
Classificação	Área Científica
OFICIAL	Ciências Biomédicas e da Vida

Ocorrência: 2022/2023 - 2S

Ativa?	Yes
Unidade Responsável:	Departamento de Sistemas e Informática
Curso/CE Responsável:

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
LTB	40	Plano de Estudos	3	-	6	60	162

Língua de trabalho

Portuguese

Objetivos

Learning outcomes include the acquisition of skills in human genetics and notions of
genetic toxicology.

Resultados de aprendizagem e competências

Learning outcomes include the acquisition of skills in human genetics, considering the role of genomics and proteomics to the more update knowledge. It is also expected that the student acquires notions of genetic toxicology understanding the dynamics of DNA lesions of the human genome caused by chemical and physical agents. Also acquisition of skills in genetic toxicology is a learning outcome, where the ability to identify mutagenic and carcinogenic agents, to understand the biological doses and protective procedures of toxic exposure of populations must be achieved. The expected outcomes of the theoreticalpractical component are acquisition of skills for data analysis from genetics and from genetic toxicology subjects. Mutation analysis and prediction of proteins damage, expression and immunostaining data and
also the prediction of biological risks of different agents such as radiation, heavy metals or phytotherapics medicines are examples of learning outcomes.

Modo de trabalho

Presencial

Programa

Theoretical component
1. Human Genetics
Notions of heredity
Chromosomes and karyotype
Mitochondrial DNA haplogroups
Repair systems

2. Molecular genetics
Genomics and proteomics
The Role of RNA
Induced mutagenesis
Cell Transfection
Expression and cell immunostaining

3. Recombinant DNA techniques
Construction of recombinant molecules
Automated sequencing methods
Applications to genetics

4. Genetic toxicology
Genotoxic effects and toxicity
Mechanisms of DNA lesions and repair systems
Mutagens and carcinogens
Genotoxic risk
Biomonitoring of populations

Theoretical-practical component

• Karyotypes and cytogenetic techniques


• Short-term tests: Ames test, chromosome aberration test, micronuclei test and sister chromatids exchange test


• Comet assay (SCGE)


• Cell culture techniques


• PCR techniques


• Electrophoresis techniques


• Induced mutagenesis techniques


• Analysis of DNA / RNA sequences


• Assessing the cancer risk


• Data analysis of cellular toxicity

Bibliografia Obrigatória

Hartl, D. and Bruce Cochrane; Analysis of Genes and Genomes, Jones and Bartlet, 2017. ISBN: ISBN: 9781284122930  
Mark F. Sanders, ; Genetic Analysis: An Integrated Approach, GlobalEdition, Pearson (Intl) , 2016. ISBN: ISBN: 9781292092362

Bibliografia Complementar

Griffiths AJF, Wessler SR et al.; An Introduction to Genetic Analysis, W. H. Freeman., 2015. ISBN: ISBN: 978-1-4641-0948-5 (https://www.ncbi.nlm.nih.gov/books/NBK21766/)
Sharbel Weidner Maluf; Mariluce Riegel; ; Citogenética Humana. , Artmed, 2011. ISBN: ISBN: 9788536324999
Tom Strachan and Andrew Read. ; Genética Molecular Humana, Artmed, 2013. ISBN: ISBN: 9788565852517
Casarett e Doull ; Fundamentos em Toxicologia, McGraw-Hill. New York, 2010. ISBN: ISBN 0071622403
James D. Watson; Richard M. Myers; Amy A. Caudy; Jan A.Witkowski; DNA Recombinante Genes e Genomas, Artmed, 2009. ISBN: 9788536313757

Métodos de ensino e atividades de aprendizagem

The teaching methodologies are based on an expository-participatory methodology, that in the theoretical component allows the use of audiovisual media and moments of discussion in class. The theoretical and practical component based on solving exercises and interpretation of experimental results using different approaches, namely different software for data analyses is designed to complement and consolidate the knowledge acquired in the theoretical contents. The evaluation of UC is continuous and involves moments of individual assessment.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	100,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	108,00
Frequência das aulas	60,00
Total:	168,00

Obtenção de frequência

Continuous assessment : two tests (50% + 50%); or final exam counting 100% if the student did not passed through continuous assessment.

(Note: in order to pass by continuous assessment the final grade must be superior to 9,5 point and evaluation moment must have a minimal grade of 8 points)

Fórmula de cálculo da classificação final

Continuous assessment: final grade = grade from 1st test/2 + grade from second test/2 or exam counting 100% if the student did not pass through continuous assessment.