Biochemistry and Biochemical Analysis

Code:

TLQB015

Sigla:

BAB

Áreas Científicas
Classificação	Área Científica
CNAEF	Biology and Biochemistry

Ocorrência: 2023/2024 - 1S

Ativa?	Yes
Unidade Responsável:	Departamento de Engenharia Química e Biológica
Curso/CE Responsável:	Professional Higher Technical Course in Chemical and Biological Laboratory Technologies

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
TLQB	15	Study Plan	2	-	7	67,5	189

Docência - Responsabilidades

Docente	Responsabilidade
Ana Cláudia Cavaco de Sousa Coelho

Docência - Horas

Theorethical and Practical :	2,00
Practical and Laboratory:	2,50

Type	Docente	Turmas	Horas
Theorethical and Practical	Totais	1	2,00
Theorethical and Practical	Ana Cláudia Cavaco de Sousa Coelho		2,00
Practical and Laboratory	Totais	1	2,50
Practical and Laboratory	Pedro da Conceição Rosado		2,50

Língua de trabalho

Portuguese
Obs.: Português suitable for english speaken students

Objetivos

At the end of the course, students are expected to have acquired theoretical knowledge about the function and structure of biomolecules belonging to the four most representative classes, such as nucleic acids (RNA and DNA), carbohydrates, lipids and proteins, and to have notions about enzyme kinetics and enzyme assays and to understand the interconnection between the different metabolic pathways and their importance for cellular homeostasis.
In addition to acquiring theoretical knowledge, students should know how to apply it in a laboratory context, including the preparation of typical buffer solutions for biochemical tests, techniques for purifying biomolecules using chromatography and ultrafiltration, dosing techniques for biomolecules (such as carbohydrates, proteins and nucleic acids), mastering colorimetric methods and spectrophotometric analyses, analytical techniques for separating proteins using acrylamide gel electrophoresis, and analysis and dosing methods based on direct and indirect enzymatic assays.

Resultados de aprendizagem e competências

Not applicable

Modo de trabalho

Presencial

Programa

Theoretical Contents:

Biodiversity of living organisms; different types of cells and their characteristics

Function and structure of biomolecules: proteins, nucleic acids (DNA and RNA), carbohydrates, lipids.

Protein Synthesis (transcription and translation)

Enzyme kinetics (specific enzymatic activity, Michaelis Menten kinetics, enzymatic assays)

Laboratory Application Contents

Spectrophotometric methods: determination of nucleic acids or proteins by UV absorption and by quantitative colorimetric methods.

Preparative methods of separating biomolecules:

Dialysis; centrifugation, ultracentrifugation and gradient centrifugation.

Filtration and ultrafiltration techniques and chromatographic methods: Hydrophobic, ion exchange, affinity and molecular exclusion interactions. Application in the isolation and purification of proteins and nucleic acids.

Electrophoretic methods. Separation of proteins on an SDS-PAGE gel and nucleic acids on an agarose gel.

3. Determination of specific enzymatic activity and kinetic parameters of Michaelis Menten. Determination of enzymatic activity and quantification methods through direct and indirect enzymatic assays

Bibliografia Obrigatória

D. L. Nelson; M. M. Cox ; Lehninger´s Principles of Biochemistry, W. H. Freeman and Company, 2012. ISBN: 9780716743392

Bibliografia Complementar

J. M. Berg; J. L. Tymoczko; L. Stryer ; Biochemistry, Stryer , W. H. Freeman and Company, 2006. ISBN: 9780716767664
D. Voet ; Biochemistry , Voet , Wiley, John Wiley & Sons, 2010. ISBN: 978-0470570951
A. Quintas ; Bioquímica : Organização Molecular da Vida , Lidel, 2008. ISBN: 978972757431

Métodos de ensino e atividades de aprendizagem

The Curricular Unit assessment is considered as a whole, integrating the theoretical and practical component. In the theoretical component, an expository-participatory methodology is used, prioritizing student participation. Theoretical-practical classes will allow to foster the students' ability to work independently in the resolution of exercise sheets on the themes presented in the theoretical classes. In the laboratory practical component, experimental works are developed that cover the contents of the UC and aim to provide the student with skills in the scope of “know-how”. The theoretical/theoretical-practical component is evaluated through tests during the semester, and the works are evaluated through the elaboration of reports using updated bibliography and reference. The theoretical component has a weight of 50% and the practical component another 50% in the global assessment.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	50,00
Trabalho laboratorial	50,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	127,50
Frequência das aulas	30,00
Trabalho laboratorial	37,50
Total:	195,00

Obtenção de frequência

Mandatory attendance of 80% in the laboratory component.

Fórmula de cálculo da classificação final

Theoretical component (TC)

TC: (rade of the 1st test+ grade of the 2nd test)/2

Minimum mark for the average of the two tests 8 values

Laboratorial Component (LC)

LC: 0.20 x classroom context +0.50 x Assignment 1 + 0.30 x Assignment 2

Minimum mark for the laboratory component 9.5 values

Final Grade (FG)

Continuous evaluation:

FG = 50 % (TC) + 50% (LC)

Exam only for the theoretical component (TC)

FG= 50% Exam + 50% (LC)

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