Biochemistry and Biochemical Analysis

Code:

TLQBVF15

Sigla:

BBA

Áreas Científicas
Classificação	Área Científica
CNAEF	Biology and Biochemistry

Ocorrência: 2022/2023 - 1S

Ativa?	Yes
Unidade Responsável:	Departamento de Engenharia Química e Biológica
Curso/CE Responsável:

Ciclos de Estudo/Cursos

Sigla	Nº de Estudantes	Plano de Estudos	Anos Curriculares	Créditos UCN	Créditos ECTS	Horas de Contacto	Horas Totais
TLQBVF	3	Study Plan	2	-	7,5	75	202,5

Docência - Responsabilidades

Docente	Responsabilidade
Ana Cláudia Cavaco de Sousa Coelho

Docência - Horas

Theorethical and Practical :

5,00

Type	Docente	Turmas	Horas
Theorethical and Practical	Totais	1	5,00
	David Leitão da Silva		2,50
	Ana Cláudia Cavaco de Sousa Coelho		1,25

A ficha foi alterada no dia 2022-12-02.

Campos alterados: Fórmula de cálculo da classificação final

Língua de trabalho

Portuguese
Obs.: Português suitable for english speaking students

Objetivos

At the end of the curricular unit (UC) the student is expected to have theoretical knowledge about: - the structure and organization of the different cellular constituents in the different types of cells; - the function and structure of the most important biomolecules, namely, nucleic acids (RNA, DNA), carbohydrates, lipids and proteins; have notions of enzymatic kinetics and enzymatic assays.

In addition to acquiring theoretical knowledge, the student should know how to apply it in a laboratory context: - preparation of typical buffer solutions for biochemical assays, - biomolecule purification techniques, namely chromatographic and ultrafiltration techniques, - biomolecule assay techniques (carbohydrates, proteins, nucleic acids), mastering colorimetric methods and spectrophotometric analyses, - analytical techniques for protein separation (acrylamide gel electrophoresis), - analysis and assay methods based on direct and indirect enzymatic assays.

Resultados de aprendizagem e competências

Not applicable

Modo de trabalho

Presencial

Programa

Theoretical Contents:

1.Biodiversity of living organisms; different types of cells and their characteristics

2.Function and structure of biomolecules: proteins, nucleic acids (DNA and RNA), carbohydrates, lipids.

3. Protein Synthesis (transcription and translation)

4.Enzyme kinetics (specific enzyme activity, Michaelis Menten kinetics, enzyme assays)

Laboratory Application Contents

1.Spectrophotometric methods: determination of nucleic acids or proteins by UV absorption and by quantitative colorimetric methods.

2. Preparative methods of separation of biomolecules:
Dialysis; centrifugation, ultracentrifugation and gradient centrifugation.
Filtration and ultrafiltration techniques and chromatographic methods: Hydrophobic interaction, ion exchange, affinity and molecular exclusion. Application in the isolation and purification of proteins and nucleic acids.
Electrophoretic methods. Separation of proteins on SDS-PAGE gel and nucleic acids on agarose gel.

3. Determination of specific enzymatic activity and kinetic parameters of Michaelis Menten. Determination of enzymatic activity and quantification methods through direct and indirect enzymatic assays.

Bibliografia Obrigatória

D. L. Nelson; M. M. Cox; Lehninger´s Principles of Biochemistry, W. H. Freeman and Company, 2012. ISBN: 9780716743392
J. M. Berg; J. L. Tymoczko; L. Stryer; Biochemistry, Stryer, W. H. Freeman and Company, 2006. ISBN: 9780716767664

Bibliografia Complementar

A. Quintas; Bioquímica: Organização Molecular da Vid, Lidel, 2008. ISBN: 978972757431
D. Voet; Biochemistry, Voet, Wiley, John Wiley & Sons, 2010. ISBN: 978-0470570951

Métodos de ensino e atividades de aprendizagem

The UC assessment is considered as a whole, integrating the theoretical and practical components. In the theoretical component, an expository-participative methodology is used, privileging the contribution of students. Theoretical-practical classes will encourage students' ability to work independently in solving exercise sheets on the themes presented in the theoretical classes.
In the laboratory practical component, experimental works are developed that cover the contents of the UC and aim to provide the student with skills in the scope of "know-how".

The theoretical/theoretical-practical component is evaluated through tests during the semester, and the experimental work is evaluated through the elaboration of reports using updated and reference bibliography. The theoretical component has a weight of 50% and the practical component another 50% in the overall assessment.

Tipo de avaliação

Distributed evaluation with final exam

Componentes de Avaliação

Designation	Peso (%)
Teste	50,00
Trabalho laboratorial	50,00
Total:	100,00

Componentes de Ocupação

Designation	Tempo (Horas)
Estudo autónomo	127,50
Frequência das aulas	37,50
Trabalho laboratorial	37,50
Total:	202,50

Obtenção de frequência

Mandatory attendance of 80% in the laboratorial component.

Fórmula de cálculo da classificação final

Theoretical component (TC)

TC: (rade of the 1st test+ grade of the 2nd test)/2

Laboratorial Component (LC)

LC: 0.10 x classroom context +0.25 x Assignment 1 + 0.15 x Assignment 2

Final Grade (FG)

Continuous evaluation:

FG = 50 % (TC) + 50% (LC)

Exam only for the theoretical component (TC)

Evaluation by Exam:

FG= 50% Exam + 50% (LC)

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